Anion species-triggered antibody separation system utilizing a thermo-responsive polymer column under optimized constant temperature

• Anion species-triggered antibody separation under optimized constant temperature. species-responsive retention of antibodies by thermo-resopnsive polymer column. Successful therapeutic monoclonal from contaminants. Although the field drugs has grown larger, production still faces several challenges. Effective purification is required, but conventional method for cost intensive and often causes aggregation problems, indicating need new alternative methods. In present study, a temperature system use with thermo-responsive column was developed based on switching anion species in eluents. By adjusting each antibody, enabled antibodies, rituximab trastuzumab, contaminants without changing salt concentration or pH The hydrogel-modified packing material synthesized introducing n -butyl methacrylate, acrylic acid, N , ′-methylenebisacrylamide -isopropylacrylamide to surface silica beads an initiator graft-from approach. Elution behavior three types anions, such as citrate, phosphate, chloride were tested different conditions. It demonstrated that hydrogel grafted showed switchable at concentration, adsorption NaCl eluent desorption citric acid buffer eluent.